The primary structure of staphylococcal enterotoxin B. II. Isolation, composition, and sequence of chymotryptic peptides.

Oxidized and reduced carboxymethylated enterotoxin B was digested with chymotrypsin. The peptides were fractionated on AGl-X2 and Dowex 5OW-X2, and further purified by paper chromatography and electrophoresis. A total of 67 pure peptides was isolated and their sequences were determined completely or partially. These peptides provide important overlaps of tryptic peptides previously isolated and sequenced. Since most of the overlaps involve only a single residue, the complete primary structure of enterotoxin B could not be elucidated.